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- Title
Krp1, a novel kelch related protein that is involved in pseudopod elongation in transformed cells.
- Authors
Spence, H J; Johnston, I; Ewart, K; Buchanan, S J; Fitzgerald, U; Ozanne, B W
- Abstract
We have previously shown that the transcription factor AP-1 regulates the expression of genes which allow neoplastically transformed rat fibroblasts to become invasive. Searches for further AP-1 target genes led to the identification of a gene encoding a novel rat kelch family member, named kelch related protein 1 (Krp1). Kelch family members are characterized by a series of repeats at their carboxyl terminus and a BTB/POZ domain near their amino terminus. Rat Krp1 has a primarily cytoplasmic localization, and a small fraction appears to accumulate and co-localize with F-actin at membrane ruffle-like structures in the tips of pseudopodia. Overexpression of Krp1 in transformed rat fibroblasts led to the formation of dramatically elongated pseudopodia, while expression of truncated Krp1 polypeptides resulted in a reduction in the length of pseudopodia. We propose that the transformation-specific expression of Krp1 is required for pseudopod elongation, which are structures that are required for cell motility and invasion.
- Publication
Oncogene, 2000, Vol 19, Issue 10, p1266
- ISSN
0950-9232
- Publication type
Journal Article
- DOI
10.1038/sj.onc.1203433