We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A population of very small embryonic-like (VSEL) CXCR4<sup>+</sup>SSEA-1<sup>+</sup>Oct-4<sup>+</sup> stem cells identified in adult bone marrow.
- Authors
Kucia, M.; Reca, R.; Campbell, F. R.; Zuba-Surma, E.; Majka, M.; Ratajczak, J.; Ratajczak, M. Z.
- Abstract
By employing multiparameter sorting, we identified in murine bone marrow (BM) a homogenous population of rare (∼0.02% of BMMNC) Sca-1+lin−CD45− cells that express by RQ-PCR and immunohistochemistry markers of pluripotent stem cells (PSC) such as SSEA-1, Oct-4, Nanog and Rex-1. The direct electronmicroscopical analysis revealed that these cells are small (∼2–4 μm), posses large nuclei surrounded by a narrow rim of cytoplasm, and contain open-type chromatin (euchromatin) that is typical for embryonic stem cells. In vitro cultures these cells are able to differentiate into all three germ-layer lineages. The number of these cells is highest in BM from young (∼1-month-old) mice and decreases with age. It is also significantly diminished in short living DBA/2J mice as compared to long living B6 animals. These cells in vitro respond strongly to SDF-1, HGF/SF and LIF and express CXCR4, c-met and LIF-R, respectively, and since they adhere to fibroblasts they may be coisolated with BM adherent cells. We hypothesize that this population of Sca-1+lin−CD45− very small embryonic-like (VSEL) stem cells is deposited early during development in BM and could be a source of pluripotent stem cells for tissue/organ regeneration.Leukemia (2006) 20, 857–869. doi:10.1038/sj.leu.2404171; published online 23 February 2006
- Publication
Leukemia (08876924), 2006, Vol 20, Issue 5, p857
- ISSN
0887-6924
- Publication type
Academic Journal
- DOI
10.1038/sj.leu.2404171