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- Title
Internal tandem duplication of the FLT3 gene and clinical evaluation in childhood acute myeloid leukemia. The Children's Cancer and Leukemia Study Group, Japan.
- Authors
Iwai, T; Yokota, S; Nakao, M; Okamoto, T; Taniwaki, M; Onodera, N; Watanabe, A; Kikuta, A; Tanaka, A; Asami, K; Sekine, I; Mugishima, H; Nishimura, Y; Koizumi, S; Horikoshi, Y; Mimaya, J; Ohta, S; Nishikawa, K; Iwai, A; Shimokawa, T; Nakayama, M; Kawakami, K; Gushiken, T; Hyakuna, N; Fujimoto, T
- Abstract
We analyzed tandem duplication in the juxtamembrane (JM) domain of the FLT3 (FMS-like tyrosine kinase 3/FLK2, CD135) gene in 94 children with acute myeloid leukemia (AML) and evaluated its correlation with clinical features. Longer polymerase chain reaction (PCR) products were observed in five patients; 1/3 of M0, 119 of M1, 1/39 of M2, 1/9 of M3 and 1/12 of M5. The sequence analyses of abnormal PCR products showed that all the abnormal products were derived from tandem duplications involving the JM domain and that all the lengthened sequences were in-frame as we previously reported. Statistical analyses revealed a significantly lower incidence of the tandem duplication in childhood AML patients than in adult patients (P < 0.05), and significantly shorter disease-free survival in patients with mutant FLT3 than in patients with wild-type FLT3 (P < 0.05). Our results suggest that the tandem duplication in the JM domain of the FLT3 gene is not a frequent phenomenon but might be a factor of poor prognosis in childhood patients with AML.
- Publication
Leukemia, 1999, Vol 13, Issue 1, p38
- ISSN
0887-6924
- Publication type
Journal Article
- DOI
10.1038/sj.leu.2401241