We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The IgE-reactive autoantigen Hom s 2 induces damage of respiratory epithelial cells and keratinocytes via induction of IFN-gamma.
- Authors
Mittermann, Irene; Reininger, Renate; Zimmermann, Maya; Gangl, Katharina; Reisinger, Jürgen; Aichberger, Karl J; Greisenegger, Elli K; Niederberger, Verena; Seipelt, Joachim; Bohle, Barbara; Kopp, Tamara; Akdis, Cezmi A; Spitzauer, Susanne; Valent, Peter; Valenta, Rudolf
- Abstract
Hom s 2, the alpha-chain of the nascent polypeptide-associated complex, is an intracellular autoantigen that has been identified with IgE autoantibodies from atopic dermatitis patients. We investigated the humoral and cellular immune response to purified recombinant Hom s 2 (rHom s 2). rHom s 2 exhibited IgE reactivity comparable to exogenous allergens, but did not induce relevant basophil cell degranulation. The latter may be attributed to the fact that patients recognized single epitopes on Hom s 2 as revealed by IgE epitope mapping with rHom s 2 fragments. In contrast to exogenous allergens, rHom s 2 had the intrinsic ability to induce the release of IFN-gamma in cultured peripheral blood mononuclear cells from atopic as well as non-atopic individuals. IFN-gamma-containing culture supernatants from Hom s 2-stimulated peripheral blood mononuclear cells caused disintegration of respiratory epithelial cell layers and apoptosis of skin keratinocytes, which could be inhibited with a neutralizing anti-IFN-gamma antibody. Our data demonstrate that the Hom s 2 autoantigen can cause IFN-gamma-mediated cell damage.
- Publication
The Journal of investigative dermatology, 2008, Vol 128, Issue 6, p1451
- ISSN
1523-1747
- Publication type
Journal Article
- DOI
10.1038/sj.jid.5701195