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- Title
RNA editing of the microRNA-151 precursor blocks cleavage by the Dicer-TRBP complex.
- Authors
Kawahara, Yukio; Zinshteyn, Boris; Chendrimada, Thimmaiah P; Shiekhattar, Ramin; Nishikura, Kazuko
- Abstract
MicroRNAs (miRNAs) mediate translational repression or degradation of their target messenger RNAs by RNA interference (RNAi). The primary transcripts of miRNA genes (pri-miRNAs) are sequentially processed by the nuclear Drosha-DGCR8 complex to approximately 60-70 nucleotide (nt) intermediates (pre-miRNAs) and then by the cytoplasmic Dicer-TRBP complex to approximately 20-22 nt mature miRNAs. Certain pri-miRNAs are subject to RNA editing that converts adenosine to inosine (A --> I RNA editing); however, the fate of edited pri-miRNAs is mostly unknown. Here, we provide evidence that RNA editing of pri-miR-151 results in complete blockage of its cleavage by Dicer and accumulation of edited pre-miR-151 RNAs. Our results indicate that A --> I conversion at two specific positions of the pre-miRNA foldback structure can affect its interaction with the Dicer-TRBP complex, showing a new regulatory role of A --> I RNA editing in miRNA biogenesis.
- Publication
EMBO reports, 2007, Vol 8, Issue 8, p763
- ISSN
1469-221X
- Publication type
Journal Article
- DOI
10.1038/sj.embor.7401011