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- Title
Involvement of calcineurin-dependent degradation of Yap1p in Ca<sup>2+</sup>-induced G<sub>2</sub> cell-cycle regulation in Saccharomyces cerevisiae.
- Authors
Yokoyama, Hiroshi; Mizunuma, Masaki; Okamoto, Michiyo; Yamamoto, Josuke; Hirata, Dai; Miyakawa, Tokichi
- Abstract
The Ca2+-activated pathways in Saccharomyces cerevisiae induce a delay in the onset of mitosis through the activation of Swe1p, a negative regulatory kinase that inhibits the Cdc28p/Clb complex. We isolated the YAP1 gene as a multicopy suppressor of calcium sensitivity owing to the loss of ZDS1, a negative regulator of SWE1 and CLN2 gene expression. YAP1 deletion on a zds1Δ background exacerbated the Ca2+-related phenotype. Yap1p was degraded in a calcineurin-dependent manner when cells were exposed to calcium. In yap1Δ cells, the expression level of the RPN4 gene encoding a transcription factor for the subunits of the ubiquitin–proteasome system was diminished. The deletion of YAP1 gene or RPN4 gene led to the accumulation of Swe1p and Cln2p. Yap1p was a substrate of calcineurin in vivo and in vitro. The calcineurin-mediated Yap1p degradation seems to be a long adaptive response that assures a G2 delay in response to a stress that causes the activation of the calcium signalling pathways.
- Publication
EMBO Reports, 2006, Vol 7, Issue 5, p519
- ISSN
1469-221X
- Publication type
Academic Journal
- DOI
10.1038/sj.embor.7400647