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- Title
ATGL has a key role in lipid droplet/adiposome degradation in mammalian cells.
- Authors
Smirnova, Elena; Goldberg, Elysa B; Makarova, Kira S; Lin, Lin; Brown, William J; Jackson, Catherine L
- Abstract
Lipid droplets (LDs), also called adiposomes, are found in many eukaryotic cells, and are highly upregulated in lipid-storage cells, such as adipocytes. The mechanism by which adiposomes and their component neutral lipids are degraded is an important health issue with the rapidly spreading epidemic of obesity. Recently, a novel triglyceride lipase (adipose triglyceride lipase (ATGL)) that catalyses the initial step in triglyceride hydrolysis in adipocyte LDs was identified. Here, we show that ATGL also functions in non-adipocyte cells, and has an important role in LD degradation in these cells. Overexpression of wild-type ATGL causes a marked decrease in LD size, whereas a catalytically inactive mutant retains the ability to localize to LDs, but is unable to decrease their size. Depletion of ATGL by RNA interference leads to a significant increase in the size of LDs. These results show that ATGL has an important role in LD/adiposome turnover in mammalian cells.
- Publication
EMBO reports, 2006, Vol 7, Issue 1, p106
- ISSN
1469-221X
- Publication type
Journal Article
- DOI
10.1038/sj.embor.7400559