We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The PX-BAR membrane-remodeling unit of sorting nexin 9.
- Authors
Pylypenko, Olena; Lundmark, Richard; Rasmuson, Erika; Carlsson, Sven R; Rak, Alexey
- Abstract
Sorting nexins (SNXs) form a family of proteins known to interact with components in the endosomal system and to regulate various steps of vesicle transport. Sorting nexin 9 (SNX9) is involved in the late stages of clathrin-mediated endocytosis in non-neuronal cells, where together with the GTPase dynamin, it participates in the formation and scission of the vesicle neck. We report here crystal structures of the functional membrane-remodeling unit of SNX9 and show that it efficiently tubulates lipid membranes in vivo and in vitro. Elucidation of the protein superdomain structure, together with mutational analysis and biochemical and cell biological experiments, demonstrated how the SNX9 PX and BAR domains work in concert in targeting and tubulation of phosphoinositide-containing membranes. The study provides insights into the SNX9-induced membrane modulation mechanism.
- Publication
The EMBO journal, 2007, Vol 26, Issue 22, p4788
- ISSN
1460-2075
- Publication type
Journal Article
- DOI
10.1038/sj.emboj.7601889