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- Title
γ-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking.
- Authors
Neubrand, Veronika E.; Will, Rainer D.; Möbius, Wiebke; Poustka, Annemarie; Wiemann, Stefan; Schu, Peter; Dotti, Carlos G.; Pepperkok, Rainer; Simpson, Jeremy C.
- Abstract
A novel peripheral membrane protein (2c18) that interacts directly with the gamma‘ear’domain of the adaptor protein complex 1 (AP-1) in vitro and in vivo is described. Ultrastructural analysis demonstrates a colocalization of 2c18 and γ1-adaptin at the trans-Golgi network (TGN) and on vesicular profiles. Overexpression of 2c18 increases the fraction of membrane-bound γ1-adaptin and inhibits its release from membranes in response to brefeldin A. Knockdown of 2c18 reduces the steady-state levels of γ1-adaptin on membranes. Overexpression or downregulation of 2c18 leads to an increased secretion of the lysosomal hydrolase cathepsin D, which is sorted by the mannose-6-phosphate receptor at the TGN, which itself involves AP-1 function for trafficking between the TGN and endosomes. This suggests that the direct interaction of 2c18 and γ1-adaptin is crucial for membrane association and thus the function of the AP-1 complex in living cells. We propose to name this protein γ-BAR.
- Publication
EMBO Journal, 2005, Vol 24, Issue 6, p1122
- ISSN
0261-4189
- Publication type
Academic Journal
- DOI
10.1038/sj.emboj.7600600