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- Title
Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation.
- Authors
Christian M. T. Spahn, Stanley J.; Gomez- Lorenzo, Maria G.; Grassucci, Robert A.; Rene Jørgensen, Robert A.; Andersen, Gregers R.; Beckmann, Roland; Penczek, Pawel A.; Ballesta, Juan P. G.; Joachim Frank
- Abstract
An 11.7-Å-resolution cryo-EM map of the yeast 80S·eEF2 complex in the presence of the antibiotic sordarin was interpreted in molecular terms, revealing large conformational changes within eEF2 and the 80S ribosome, including a rearrangement of the functionally important ribosomal intersubunit bridges. Sordarin positions domain III of eEF2 so that it can interact with the sarcin-ricin loop of 25S rRNA and protein rpS23 (S12p). This particular conformation explains the inhibitory action of sordarin and suggests that eEF2 is stalled on the 80S ribosome in a conformation that has similarities with the GTPase activation state. A ratchet-like subunit rearrangement (RSR) occurs in the 80S·eEF2·sordarin complex that, in contrast to Escherichia coli 70S ribosomes, is also present in vacant 80S ribosomes. A model is suggested, according to which the RSR is part of a mechanism for moving the tRNAs during the translocation reaction.
- Publication
EMBO Journal, 2004, Vol 23, Issue 5, p1008
- ISSN
0261-4189
- Publication type
Academic Journal
- DOI
10.1038/sj.emboj.7600102