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- Title
Gastrointestinal handling of glycosyl [<sup>13</sup>C]ureides.
- Authors
Morrison, D J; Dodson, B; Preston, T; Weaver, L T
- Abstract
Objectives: Lactose [[SUP13]C]ureide has been proposed as a noninvasive marker for oro-caecal transit time in adults and children. The present study investigates the handling of lactose [[SUP13]C]ureide ([SUP13]C LU) and glucose [[SUP13]C]ureide ([SUP13]C GU) by the gastrointestinal tract and describes the metabolic fates of these substrates and describes the extent of tracer excretion by different routes. Study Design and Subjects: Four subjects underwent five studies in which they ingested a test meal plus (1) no substrate, (2) [SUP13]C LU, (3) [SUP13]C GU, (4) [SUP13]C LU after predosing with unlabelled lactose ureide and (5) [SUP13]C LU after predosing with glucose ureide. Subjects were studied at home with at least 1 week between tests and they all completed the study. Breath was analysed for [SUP13]CO[SUB2] recovery and urine was analysed for total [SUP13]C recovery, [SUP13]C urea recovery and [SUP13]C GU recovery. Results: The profiles and extent of tracer recovery in breath and urine were similar when either [SUP13]C GU or [SUP13]C LU was used, suggesting similar handling of these substrates by the gut. [SUP13]C GU was the major [SUP13]C-enriched species recovered in the urine even when [SUP13]C LU was consumed. Predosing with either lactose ureide or glucose ureide increased the rate of appearance of tracer, but did not alter transit times. Conclusions: [SUP13]C LU is hydrolysed to [SUP13]C GU in the small intestine with the fraction of [SUP13]C GU appearing in the urine probably limited by small intestinal permeability. Either [SUP13]C LU or [SUP13]C GU can be used to measure oro-caecal transit time. Sponsorship: Bell College, Hamilton and Chief Scientist Office, Scotland, UK.
- Publication
European Journal of Clinical Nutrition, 2003, Vol 57, Issue 8, p1017
- ISSN
0954-3007
- Publication type
Academic Journal
- DOI
10.1038/sj.ejcn.1601637