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- Title
The structure of a resuscitation-promoting factor domain from Mycobacterium tuberculosis shows homology to lysozymes.
- Authors
Cohen-Gonsaud, Martin; Barthe, Philippe; Bagnéris, Claire; Henderson, Brian; Ward, John; Roumestand, Christian; Keep, Nicholas H
- Abstract
Resuscitation-promoting factor (RPF) proteins reactivate stationary-phase cultures of (G+C)-rich Gram-positive bacteria including the causative agent of tuberculosis, Mycobacterium tuberculosis. We report the solution structure of the RPF domain from M. tuberculosis Rv1009 (RpfB) solved by heteronuclear multidimensional NMR. Structural homology with various glycoside hydrolases suggested that RpfB cleaved oligosaccharides. Biochemical studies indicate that a conserved active site glutamate is important for resuscitation activity. These data, as well as the presence of a clear binding pocket for a large molecule, indicate that oligosaccharide cleavage is probably the signal for revival from dormancy.
- Publication
Nature structural & molecular biology, 2005, Vol 12, Issue 3, p270
- ISSN
1545-9993
- Publication type
Journal Article
- DOI
10.1038/nsmb905