We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
PYM binds the cytoplasmic exon-junction complex and ribosomes to enhance translation of spliced mRNAs.
- Authors
Diem, Michael D; Chan, Chia C; Younis, Ihab; Dreyfuss, Gideon
- Abstract
Messenger RNAs produced by splicing are translated more efficiently than those produced from similar intronless precursor mRNAs (pre-mRNAs). The exon-junction complex (EJC) probably mediates this enhancement; however, the specific link between the EJC and the translation machinery has not been identified. The EJC proteins Y14 and magoh remain bound to spliced mRNAs after their export from the nucleus to the cytoplasm and are removed only when these mRNAs are translated. Here we show that PYM, a 29-kDa protein that binds the Y14-magoh complex in the cytoplasm, also binds, via a separate domain, to the small (40S) ribosomal subunit and the 48S preinitiation complex. Furthermore, PYM knockdown reduces the translation efficiency of a reporter protein produced from intron-containing, but not intronless, pre-mRNA. We suggest that PYM functions as a bridge between EJC-bearing spliced mRNAs and the translation machinery to enhance translation of the mRNAs.
- Publication
Nature structural & molecular biology, 2007, Vol 14, Issue 12, p1173
- ISSN
1545-9985
- Publication type
Journal Article
- DOI
10.1038/nsmb1321