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- Title
Dynamics of the unbound head during myosin V processive translocation.
- Authors
Dunn, Alexander R; Spudich, James A
- Abstract
Myosin V moves cargoes along actin filaments by walking hand over hand. Although numerous studies support the basic hand-over-hand model, little is known about the fleeting intermediate that occurs when the rear head detaches from the filament. Here we use submillisecond dark-field imaging of gold nanoparticle-labeled myosin V to directly observe the free head as it releases from the actin filament, diffuses forward and rebinds. We find that the unbound head rotates freely about the lever-arm junction, a trait that likely facilitates travel through crowded actin meshworks.
- Publication
Nature structural & molecular biology, 2007, Vol 14, Issue 3, p246
- ISSN
1545-9993
- Publication type
Journal Article
- DOI
10.1038/nsmb1206