We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Nucleophosmin is selectively deposited on mRNA during polyadenylation.
- Authors
Palaniswamy, Viswanathan; Moraes, Karen C M; Wilusz, Carol J; Wilusz, Jeffrey
- Abstract
Nucleophosmin (NPM), an abundant, predominantly nucleolar protein that influences numerous cellular processes, was shown to specifically associate with the bodies of messenger RNAs as a result of the process of 3'-end formation. NPM deposition requires polyadenylation but not the 3' cleavage event to occur on the transcript. Furthermore, the protein does not associate with RNAs bearing a preformed poly(A) tail or with mRNAs that have undergone cleavage but not polyadenylation. A region within 10 bases upstream of the AAUAAA element is required for NPM association, but deposition of the protein seems to be sequence independent. NPM association with poly(A)(+) mRNAs was also demonstrated in vivo. NPM, therefore, represents a mark left on transcripts as a result of 3'-end processing and may have a role in one or more of a variety of post-transcriptional processes influenced by the polyadenylation event.
- Publication
Nature structural & molecular biology, 2006, Vol 13, Issue 5, p429
- ISSN
1545-9993
- Publication type
Journal Article
- DOI
10.1038/nsmb1080