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- Title
Ndc10 is a platform for inner kinetochore assembly in budding yeast.
- Authors
Cho, Uhn-Soo; Harrison, Stephen C
- Abstract
Kinetochores link centromeric DNA to spindle microtubules and ensure faithful chromosome segregation during mitosis. In point-centromere yeasts, the CBF3 complex Skp1-Ctf13-(Cep3)2-(Ndc10)2 recognizes a conserved centromeric DNA element through contacts made by Cep3 and Ndc10. We describe here the five-domain organization of Kluyveromyces lactis Ndc10 and the structure at 2.8 Å resolution of domains I-II (residues 1-402) bound to DNA. The structure resembles tyrosine DNA recombinases, although it lacks both endonuclease and ligase activities. Structural and biochemical data demonstrate that each subunit of the Ndc10 dimer binds a separate fragment of DNA, suggesting that Ndc10 stabilizes a DNA loop at the centromere. We describe in vitro association experiments showing that specific domains of Ndc10 interact with each of the known inner-kinetochore proteins or protein complexes in budding yeast. We propose that Ndc10 provides a central platform for inner-kinetochore assembly.
- Publication
Nature Structural & Molecular Biology, 2012, Vol 19, Issue 1, p48
- ISSN
1545-9993
- Publication type
Academic Journal
- DOI
10.1038/nsmb.2178