We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs.
- Authors
Garcia, David M; Baek, Daehyun; Shin, Chanseok; Bell, George W; Grimson, Andrew; Bartel, David P
- Abstract
Most metazoan microRNAs (miRNAs) target many genes for repression, but the nematode lsy-6 miRNA is much less proficient. Here we show that the low proficiency of lsy-6 can be recapitulated in HeLa cells and that miR-23, a mammalian miRNA, also has low proficiency in these cells. Reporter results and array data indicate two properties of these miRNAs that impart low proficiency: their weak predicted seed-pairing stability (SPS) and their high target-site abundance (TA). These two properties also explain differential propensities of small interfering RNAs (siRNAs) to repress unintended targets. Using these insights, we expand the TargetScan tool for quantitatively predicting miRNA regulation (and siRNA off-targeting) to model differential miRNA (and siRNA) proficiencies, thereby improving prediction performance. We propose that siRNAs designed to have both weaker SPS and higher TA will have fewer off-targets without compromised on-target activity.
- Publication
Nature structural & molecular biology, 2011, Vol 18, Issue 10, p1139
- ISSN
1545-9985
- Publication type
Journal Article
- DOI
10.1038/nsmb.2115