We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Crystal structure of γ-tubulin complex protein GCP4 provides insight into microtubule nucleation.
- Authors
Guillet, Valérie; Knibiehler, Martine; Gregory-Pauron, Lynn; Remy, Marie-Hélène; Chemin, Cécile; Raynaud-Messina, Brigitte; Bon, Cécile; Kollman, Justin M; Agard, David A; Merdes, Andreas; Mourey, Lionel
- Abstract
Microtubule nucleation in all eukaryotes involves γ-tubulin small complexes (γTuSCs) that comprise two molecules of γ-tubulin bound to γ-tubulin complex proteins (GCPs) GCP2 and GCP3. In many eukaryotes, multiple γTuSCs associate with GCP4, GCP5 and GCP6 into large γ-tubulin ring complexes (γTuRCs). Recent cryo-EM studies indicate that a scaffold similar to γTuRCs is formed by lateral association of γTuSCs, with the C-terminal regions of GCP2 and GCP3 binding γ-tubulin molecules. However, the exact role of GCPs in microtubule nucleation remains unknown. Here we report the crystal structure of human GCP4 and show that its C-terminal domain binds directly to γ-tubulin. The human GCP4 structure is the prototype for all GCPs, as it can be precisely positioned within the γTuSC envelope, revealing the nature of protein-protein interactions and conformational changes regulating nucleation activity.
- Publication
Nature structural & molecular biology, 2011, Vol 18, Issue 8, p915
- ISSN
1545-9985
- Publication type
Journal Article
- DOI
10.1038/nsmb.2083