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- Title
Genome-wide identification of Ago2 binding sites from mouse embryonic stem cells with and without mature microRNAs.
- Authors
Leung, Anthony K L; Young, Amanda G; Bhutkar, Arjun; Zheng, Grace X; Bosson, Andrew D; Nielsen, Cydney B; Sharp, Phillip A
- Abstract
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer⁻/⁻ mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
- Publication
Nature structural & molecular biology, 2011, Vol 18, Issue 2, p237
- ISSN
1545-9985
- Publication type
Journal Article
- DOI
10.1038/nsmb.1991