We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
H2A.Z nucleosomes enriched over active genes are homotypic.
- Authors
Weber, Christopher M; Henikoff, Jorja G; Henikoff, Steven
- Abstract
Nucleosomes that contain the histone variant H2A.Z are enriched around transcriptional start sites, but the mechanistic basis for this enrichment is unknown. A single octameric nucleosome can contain two H2A.Z histones (homotypic) or one H2A.Z and one canonical H2A (heterotypic). To elucidate the function of H2A.Z, we generated high-resolution maps of homotypic and heterotypic Drosophila H2A.Z (H2Av) nucleosomes. Although homotypic and heterotypic H2A.Z nucleosomes mapped throughout most of the genome, homotypic nucleosomes were enriched and heterotypic nucleosomes were depleted downstream of active promoters and intron-exon junctions. The distribution of homotypic H2A.Z nucleosomes resembled that of classical active chromatin and showed evidence of disruption during transcriptional elongation. Both homotypic H2A.Z nucleosomes and classical active chromatin were depleted downstream of paused polymerases. Our results suggest that H2A.Z enrichment patterns result from intrinsic structural differences between heterotypic and homotypic H2A.Z nucleosomes that follow disruption during transcriptional elongation.
- Publication
Nature structural & molecular biology, 2010, Vol 17, Issue 12, p1500
- ISSN
1545-9985
- Publication type
Journal Article
- DOI
10.1038/nsmb.1926