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- Title
Unique functionality of 22-nt miRNAs in triggering RDR6-dependent siRNA biogenesis from target transcripts in Arabidopsis.
- Authors
Cuperus, Josh T; Carbonell, Alberto; Fahlgren, Noah; Garcia-Ruiz, Hernan; Burke, Russell T; Takeda, Atsushi; Sullivan, Christopher M; Gilbert, Sunny D; Montgomery, Taiowa A; Carrington, James C
- Abstract
RNA interference pathways can involve amplification of secondary siRNAs by RNA-dependent RNA polymerases. In plants, RDR6-dependent secondary siRNAs arise from transcripts targeted by some microRNAs (miRNAs). Here, Arabidopsis thaliana secondary siRNAs from mRNA as well as trans-acting siRNAs are shown to be triggered through initial targeting by a 22-nucleotide (nt) miRNA that associates with AGO1. In contrast to canonical 21-nt miRNAs, 22-nt miRNAs primarily arise from foldback precursors containing asymmetric bulges. Using artificial miRNA constructs, conversion of asymmetric foldbacks to symmetric foldbacks resulted in the production of 21-nt forms of miR173, miR472 and miR828. Both 21- and 22-nt forms associated with AGO1 and guided accurate slicer activity, but only 22-nt forms were competent to trigger RDR6-dependent siRNA production from target RNA. These data suggest that AGO1 functions differentially with 21- and 22-nt miRNAs to engage the RDR6-associated amplification apparatus.
- Publication
Nature structural & molecular biology, 2010, Vol 17, Issue 8, p997
- ISSN
1545-9985
- Publication type
Journal Article
- DOI
10.1038/nsmb.1866