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- Title
Structural basis for the transcriptional regulation of membrane lipid homeostasis.
- Authors
Miller, Darcie J; Zhang, Yong-Mei; Subramanian, Chitra; Rock, Charles O; White, Stephen W
- Abstract
DesT is a transcriptional repressor that regulates the genes that control the unsaturated:saturated fatty acid ratio available for membrane lipid synthesis. DesT bound to unsaturated acyl-CoA has a high affinity for its cognate palindromic DNA-binding site, whereas DesT bound to saturated acyl-CoA does not bind this site. Structural analyses of the DesT-oleoyl-CoA-DNA and DesT-palmitoyl-CoA complexes reveal that acyl chain shape directly influences the packing of hydrophobic core residues within the DesT ligand-binding domain. These changes are propagated to the paired DNA-binding domains via conformational changes to modulate DNA binding. These structural interpretations are supported by the in vitro and in vivo characterization of site-directed mutants. The regulation of DesT by the unsaturated:saturated ratio of acyl chains rather than the concentration of a single ligand is a paradigm for understanding transcriptional regulation of membrane lipid homeostasis.
- Publication
Nature structural & molecular biology, 2010, Vol 17, Issue 8, p971
- ISSN
1545-9985
- Publication type
Journal Article
- DOI
10.1038/nsmb.1847