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- Title
Structural basis of G-tract recognition and encaging by hnRNP F quasi-RRMs.
- Authors
Dominguez, Cyril; Fisette, Jean-François; Chabot, Benoit; Allain, Frédéric H-T
- Abstract
The heterogeneous nuclear ribonucleoprotein (hnRNP) F is involved in the regulation of mRNA metabolism by specifically recognizing G-tract RNA sequences. We have determined the solution structures of the three quasi-RNA-recognition motifs (qRRMs) of hnRNP F in complex with G-tract RNA. These structures show that qRRMs bind RNA in a very unusual manner, with the G-tract 'encaged', making the qRRM a novel RNA binding domain. We defined a consensus signature sequence for qRRMs and identified other human qRRM-containing proteins that also specifically recognize G-tract RNAs. Our structures explain how qRRMs can sequester G-tracts, maintaining them in a single-stranded conformation. We also show that isolated qRRMs of hnRNP F are sufficient to regulate the alternative splicing of the Bcl-x pre-mRNA, suggesting that hnRNP F would act by remodeling RNA secondary and tertiary structures.
- Publication
Nature structural & molecular biology, 2010, Vol 17, Issue 7, p853
- ISSN
1545-9985
- Publication type
Journal Article
- DOI
10.1038/nsmb.1814