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- Title
Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine.
- Authors
Newby, Meredith I; Greenbaum, Nancy L
- Abstract
Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing.
- Publication
Nature structural biology, 2002, Vol 9, Issue 12, p958
- ISSN
1072-8368
- Publication type
Journal Article
- DOI
10.1038/nsb873