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- Title
Crystal structure of the APC10/DOC1 subunit of the human anaphase-promoting complex.
- Authors
Wendt, K S; Vodermaier, H C; Jacob, U; Gieffers, C; Gmachl, M; Peters, J M; Huber, R; Sondermann, P
- Abstract
The anaphase-promoting complex (APC), or cyclosome, is a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC is composed of at least 11 subunits; no structure has been determined for any of these subunits. The subunit APC10/DOC1, a one-domain protein consisting of 185 amino acids, has a conserved core (residues 22-161) that is homologous to domains found in several other putative ubiquitin ligases and, therefore, may play a role in ubiquitination reactions. Here we report the crystal structure of human APC10 at 1.6 A resolution. The core of the protein is formed by a beta-sandwich that adopts a jellyroll fold. Unexpectedly, this structure is highly similar to ligand-binding domains of several bacterial and eukaryotic proteins, such as galactose oxidase and coagulation factor Va, raising the possibility that APC10 may function by binding a yet unidentified ligand. We further provide biochemical evidence that the C-terminus of APC10 binds to CDC27/APC3, an APC subunit that contains multiple tetratrico peptide repeats.
- Publication
Nature structural biology, 2001, Vol 8, Issue 9, p784
- ISSN
1072-8368
- Publication type
Journal Article
- DOI
10.1038/nsb0901-784