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- Title
Innovation: Stable isotope probing — linking microbial identity to function.
- Authors
Dumont, Marc G.; Murrell, J. Colin
- Abstract
Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope, such as 13C, and the identification of active microorganisms by the selective recovery and analysis of isotope-enriched cellular components. DNA and rRNA are the most informative taxonomic biomarkers and 13C-labelled molecules can be purified from unlabelled nucleic acid by density-gradient centrifugation. The future holds great promise for SIP, particularly when combined with other emerging technologies such as microarrays and metagenomics.
- Publication
Nature Reviews Microbiology, 2005, Vol 3, Issue 6, p499
- ISSN
1740-1526
- Publication type
Academic Journal
- DOI
10.1038/nrmicro1162