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- Title
RNA sequencing: advances, challenges and opportunities.
- Authors
Ozsolak, Fatih; Milos, Patrice M
- Abstract
In the few years since its initial application, massively parallel cDNA sequencing, or RNA-seq, has allowed many advances in the characterization and quantification of transcriptomes. Recently, several developments in RNA-seq methods have provided an even more complete characterization of RNA transcripts. These developments include improvements in transcription start site mapping, strand-specific measurements, gene fusion detection, small RNA characterization and detection of alternative splicing events. Ongoing developments promise further advances in the application of RNA-seq, particularly direct RNA sequencing and approaches that allow RNA quantification from very small amounts of cellular materials.
- Publication
Nature reviews. Genetics, 2011, Vol 12, Issue 2, p87
- ISSN
1471-0064
- Publication type
Journal Article
- DOI
10.1038/nrg2934