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- Title
Evaluating genome-scale approaches to eukaryotic DNA replication.
- Authors
Gilbert, David M
- Abstract
Mechanisms regulating where and when eukaryotic DNA replication initiates remain a mystery. Recently, genome-scale methods have been brought to bear on this problem. The identification of replication origins and their associated proteins in yeasts is a well-integrated investigative tool, but corresponding data sets from multicellular organisms are scarce. By contrast, standardized protocols for evaluating replication timing have generated informative data sets for most eukaryotic systems. Here, I summarize the genome-scale methods that are most frequently used to analyse replication in eukaryotes, the kinds of questions each method can address and the technical hurdles that must be overcome to gain a complete understanding of the nature of eukaryotic replication origins.
- Publication
Nature reviews. Genetics, 2010, Vol 11, Issue 10, p673
- ISSN
1471-0064
- Publication type
Journal Article
- DOI
10.1038/nrg2830