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- Title
ChIP-seq: advantages and challenges of a maturing technology.
- Authors
Park, Peter J
- Abstract
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a technique for genome-wide profiling of DNA-binding proteins, histone modifications or nucleosomes. Owing to the tremendous progress in next-generation sequencing technology, ChIP-seq offers higher resolution, less noise and greater coverage than its array-based predecessor ChIP-chip. With the decreasing cost of sequencing, ChIP-seq has become an indispensable tool for studying gene regulation and epigenetic mechanisms. In this Review, I describe the benefits and challenges in harnessing this technique with an emphasis on issues related to experimental design and data analysis. ChIP-seq experiments generate large quantities of data, and effective computational analysis will be crucial for uncovering biological mechanisms.
- Publication
Nature reviews. Genetics, 2009, Vol 10, Issue 10, p669
- ISSN
1471-0064
- Publication type
Journal Article
- DOI
10.1038/nrg2641