We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Asymmetric membrane ganglioside sialidase activity specifies axonal fate.
- Authors
Da Silva, Jorge Santos; Hasegawa, Takafumi; Miyagi, Taeko; Dotti, Carlos G; Abad-Rodriguez, Jose
- Abstract
Axon specification triggers the polarization of neurons and requires the localized destabilization of filamentous actin. Here we show that plasma membrane ganglioside sialidase (PMGS) asymmetrically accumulates at the tip of one neurite of the unpolarized rat neuron, inducing actin instability. Suppressing PMGS activity blocks axonal generation, whereas stimulating it accelerates the formation of a single (not several) axon. PMGS induces axon specification by enhancing TrkA activity locally, which triggers phosphatidylinositol-3-kinase (PI3K)- and Rac1-dependent inhibition of RhoA signaling and the consequent actin depolymerization in one neurite only. Thus, spatial restriction of an actin-regulating molecular machinery, in this case a membrane enzymatic activity, before polarization is enough to determine axonal fate.
- Publication
Nature neuroscience, 2005, Vol 8, Issue 5, p606
- ISSN
1097-6256
- Publication type
Journal Article
- DOI
10.1038/nn1442