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- Title
Neuronal activity modifies the DNA methylation landscape in the adult brain.
- Authors
Guo, Junjie U; Ma, Dengke K; Mo, Huan; Ball, Madeleine P; Jang, Mi-Hyeon; Bonaguidi, Michael A; Balazer, Jacob A; Eaves, Hugh L; Xie, Bin; Ford, Eric; Zhang, Kun; Ming, Guo-li; Gao, Yuan; Song, Hongjun
- Abstract
DNA methylation has been traditionally viewed as a highly stable epigenetic mark in postmitotic cells. However, postnatal brains appear to show stimulus-induced methylation changes, at least in a few identified CpG dinucleotides. How extensively the neuronal DNA methylome is regulated by neuronal activity is unknown. Using a next-generation sequencing-based method for genome-wide analysis at single-nucleotide resolution, we quantitatively compared the CpG methylation landscape of adult mouse dentate granule neurons in vivo before and after synchronous neuronal activation. About 1.4% of 219,991 CpGs measured showed rapid active demethylation or de novo methylation. Some modifications remained stable for at least 24 h. These activity-modified CpGs showed a broad genomic distribution with significant enrichment in low-CpG density regions, and were associated with brain-specific genes related to neuronal plasticity. Our study implicates modification of the neuronal DNA methylome as a previously underappreciated mechanism for activity-dependent epigenetic regulation in the adult nervous system.
- Publication
Nature neuroscience, 2011, Vol 14, Issue 10, p1345
- ISSN
1546-1726
- Publication type
Journal Article
- DOI
10.1038/nn.2900