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- Title
A highly sensitive protein-protein interaction assay based on Gaussia luciferase.
- Authors
Remy, Ingrid; Michnick, Stephen W
- Abstract
Protein-fragment complementation assays (PCAs) provide a general strategy to study the dynamics of protein-protein interactions in vivo and in vitro. The full potential of PCA requires assays that are fully reversible and sensitive at subendogenous protein expression levels. We describe a new assay that meets these criteria, based on the Gaussia princeps luciferase enzyme, demonstrating chemical reversal, and induction and inhibition of a key interaction linking insulin and TGFbeta signaling.
- Publication
Nature methods, 2006, Vol 3, Issue 12, p977
- ISSN
1548-7091
- Publication type
Journal Article
- DOI
10.1038/nmeth979