We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Accurate phylogenetic classification of variable-length DNA fragments.
- Authors
McHardy, Alice Carolyn; Martín, Héctor García; Tsirigos, Aristotelis; Hugenholtz, Philip; Rigoutsos, Isidore
- Abstract
Metagenome studies have retrieved vast amounts of sequence data from a variety of environments leading to new discoveries and insights into the uncultured microbial world. Except for very simple communities, the encountered diversity has made fragment assembly and the subsequent analysis a challenging problem. A taxonomic characterization of metagenomic fragments is required for a deeper understanding of shotgun-sequenced microbial communities, but success has mostly been limited to sequences containing phylogenetic marker genes. Here we present PhyloPythia, a composition-based classifier that combines higher-level generic clades from a set of 340 completed genomes with sample-derived population models. Extensive analyses on synthetic and real metagenome data sets showed that PhyloPythia allows the accurate classification of most sequence fragments across all considered taxonomic ranks, even for unknown organisms. The method requires no more than 100 kb of training sequence for the creation of accurate models of sample-specific populations and can assign fragments >or=1 kb with high specificity.
- Publication
Nature methods, 2007, Vol 4, Issue 1, p63
- ISSN
1548-7091
- Publication type
Journal Article
- DOI
10.1038/nmeth976