We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays.
- Authors
Kulkarni, Meghana M; Booker, Matthew; Silver, Serena J; Friedman, Adam; Hong, Pengyu; Perrimon, Norbert; Mathey-Prevot, Bernard
- Abstract
To evaluate the specificity of long dsRNAs used in high-throughput RNA interference (RNAi) screens performed at the Drosophila RNAi Screening Center (DRSC), we performed a global analysis of their activity in 30 genome-wide screens completed at our facility. Notably, our analysis predicts that dsRNAs containing > or = 19-nucleotide perfect matches identified in silico to unintended targets may contribute to a significant false positive error rate arising from off-target effects. We confirmed experimentally that such sequences in dsRNAs lead to false positives and to efficient knockdown of a cross-hybridizing transcript, raising a cautionary note about interpreting results based on the use of a single dsRNA per gene. Although a full appreciation of all causes of false positive errors remains to be determined, we suggest simple guidelines to help ensure high-quality information from RNAi high-throughput screens.
- Publication
Nature methods, 2006, Vol 3, Issue 10, p833
- ISSN
1548-7091
- Publication type
Journal Article
- DOI
10.1038/nmeth935