We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).
- Authors
Rust, Michael J; Bates, Mark; Zhuang, Xiaowei
- Abstract
We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.
- Publication
Nature methods, 2006, Vol 3, Issue 10, p793
- ISSN
1548-7091
- Publication type
Journal Article
- DOI
10.1038/nmeth929