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- Title
Nanoscale resolution in GFP-based microscopy.
- Authors
Willig, Katrin I; Kellner, Robert R; Medda, Rebecca; Hein, Birka; Jakobs, Stefan; Hell, Stefan W
- Abstract
We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The approximately 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.
- Publication
Nature methods, 2006, Vol 3, Issue 9, p721
- ISSN
1548-7091
- Publication type
Journal Article
- DOI
10.1038/nmeth922