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- Title
Enzymatically prepared RNAi libraries.
- Authors
Buchholz, Frank; Kittler, Ralf; Slabicki, Mikolaj; Theis, Mirko
- Abstract
Large-scale RNA interference (RNAi) screens in mammalian cells have mainly used synthetic small interfering RNA (siRNA) or short hairpin RNA (shRNA) libraries. The RNAi triggers for both of these approaches were designed with algorithm-based predictions to identify single sequences for mRNA knockdown. Alternatives to these approaches have recently been developed using enzymatic methods. Here we describe the concepts of enzymatically prepared shRNA and siRNA libraries, and discuss their strengths and limitations.
- Publication
Nature methods, 2006, Vol 3, Issue 9, p696
- ISSN
1548-7091
- Publication type
Journal Article
- DOI
10.1038/nmeth912