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- Title
In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes.
- Authors
Kloosterman, Wigard P; Wienholds, Erno; de Bruijn, Ewart; Kauppinen, Sakari; Plasterk, Ronald H A
- Abstract
MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.
- Publication
Nature methods, 2006, Vol 3, Issue 1, p27
- ISSN
1548-7091
- Publication type
Journal Article
- DOI
10.1038/nmeth843