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- Title
A guide to choosing fluorescent proteins.
- Authors
Shaner, Nathan C; Steinbach, Paul A; Tsien, Roger Y
- Abstract
The recent explosion in the diversity of available fluorescent proteins (FPs) promises a wide variety of new tools for biological imaging. With no unified standard for assessing these tools, however, a researcher is faced with difficult questions. Which FPs are best for general use? Which are the brightest? What additional factors determine which are best for a given experiment? Although in many cases, a trial-and-error approach may still be necessary in determining the answers to these questions, a unified characterization of the best available FPs provides a useful guide in narrowing down the options.
- Publication
Nature methods, 2005, Vol 2, Issue 12, p905
- ISSN
1548-7091
- Publication type
Journal Article
- DOI
10.1038/nmeth819