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- Title
Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system.
- Authors
Keng, Vincent W; Yae, Kojiro; Hayakawa, Tomoko; Mizuno, Sumi; Uno, Yoshihiro; Yusa, Kosuke; Kokubu, Chikara; Kinoshita, Taroh; Akagi, Keiko; Jenkins, Nancy A; Copeland, Neal G; Horie, Kyoji; Takeda, Junji
- Abstract
Recent consolidation of the whole-genome sequence with genome-wide transcriptome profiling revealed the existence of functional units within the genome in specific chromosomal regions, as seen in the coordinated expression of gene clusters and colocalization of functionally related genes. An efficient region-specific mutagenesis screen would greatly facilitate research in addressing the importance of these clusters. Here we use the 'local hopping' phenomenon of a DNA-type transposon, Sleeping Beauty (SB), for region-specific saturation mutagenesis. A transgenic mouse containing both transposon (acts as a mutagen) and transposase (recognizes and mobilizes the transposon) was bred for germ-cell transposition events, allowing us to generate many mutant mice. All genes within a 4-Mb region of the original donor site were mutated by SB, indicating the potential of this system for functional genomic studies within a specific chromosomal region.
- Publication
Nature methods, 2005, Vol 2, Issue 10, p763
- ISSN
1548-7091
- Publication type
Journal Article
- DOI
10.1038/nmeth795