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- Title
Highly inclined thin illumination enables clear single-molecule imaging in cells.
- Authors
Tokunaga, Makio; Imamoto, Naoko; Sakata-Sogawa, Kumiko
- Abstract
We describe a simple illumination method of fluorescence microscopy for molecular imaging. Illumination by a highly inclined and thin beam increases image intensity and decreases background intensity, yielding a signal/background ratio about eightfold greater than that of epi-illumination. A high ratio yielded clear single-molecule images and three-dimensional images using cultured mammalian cells, enabling one to visualize and quantify molecular dynamics, interactions and kinetics in cells for molecular systems biology.
- Publication
Nature methods, 2008, Vol 5, Issue 2, p159
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth1171