We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies.
- Authors
Xiaodong Zhang; Chunguang Guo; Chen, Yuetin; Shulha, Hennady P.; Schnetz, Michael P.; LaFramboise, Thomas; Bartels, Cynthia F.; Markowitz, Sanford; Weng, Zhiping; Scacheri, Peter C.; Wang, Zhenghe
- Abstract
We developed a strategy to introduce epitope tag–encoding DNA into endogenous loci by homologous recombination–mediated 'knock-in'. The tagging method is straightforward, can be applied to many loci and several human somatic cell lines, and can facilitate many functional analyses including western blot, immunoprecipitation, immunofluorescence and chromatin immunoprecipitation–microarray (ChIP-chip). The knock-in approach provides a general solution for the study of proteins to which antibodies are substandard or not available.
- Publication
Nature Methods, 2008, Vol 5, Issue 2, p163
- ISSN
1548-7091
- Publication type
Academic Journal
- DOI
10.1038/nmeth1170