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- Title
In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography.
- Authors
Vinegoni, Claudio; Pitsouli, Chrysoula; Razansky, Daniel; Perrimon, Norbert; Ntziachristos, Vasilis
- Abstract
We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time.
- Publication
Nature methods, 2008, Vol 5, Issue 1, p45
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth1149