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- Title
An FKBP destabilization domain modulates protein levels in Plasmodium falciparum.
- Authors
Armstrong, Christopher M; Goldberg, Daniel E
- Abstract
To enhance the repertoire of molecular tools for studying malaria parasite biology, we adapted a ligand-regulatable FKBP protein destabilization domain (ddFKBP) for use in P. falciparum. We destabilized the reporter yellow fluorescent protein (YFP) and the P. falciparum protease falcipain-2 in a ligand-reversible manner by tagging with ddFKBP. The swollen food vacuole phenotype of falcipain-2 knockout parasites could be rescued in a Shld1 ligand-dependent fashion by falcipain-2-ddFKBP expression.
- Publication
Nature methods, 2007, Vol 4, Issue 12, p1007
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth1132