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- Title
Direct selection of human genomic loci by microarray hybridization.
- Authors
Albert, Thomas J; Molla, Michael N; Muzny, Donna M; Nazareth, Lynne; Wheeler, David; Song, Xingzhi; Richmond, Todd A; Middle, Chris M; Rodesch, Matthew J; Packard, Charles J; Weinstock, George M; Gibbs, Richard A
- Abstract
We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions.
- Publication
Nature methods, 2007, Vol 4, Issue 11, p903
- ISSN
1548-7091
- Publication type
Journal Article
- DOI
10.1038/nmeth1111