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- Title
Comprehensive analysis of diverse ribonucleoprotein complexes.
- Authors
Oeffinger, Marlene; Wei, Karen E; Rogers, Richard; DeGrasse, Jeffrey A; Chait, Brian T; Aitchison, John D; Rout, Michael P
- Abstract
The study of the dynamic interactome of cellular ribonucleoprotein (RNP) particles has been hampered by severe methodological limitations. In particular, the affinity purification of intact RNP complexes from cell lysates suffers from RNA degradation, loss of interacting macromolecules and poor overall yields. Here we describe a rapid affinity-purification method for efficient isolation of the subcomplexes that dynamically organize different RNP biogenesis pathways in Saccharomyces cerevisiae. Our method overcomes many of the previous limitations to produce large RNP interactomes with almost no contamination.
- Publication
Nature methods, 2007, Vol 4, Issue 11, p951
- ISSN
1548-7091
- Publication type
Journal Article
- DOI
10.1038/nmeth1101