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- Title
An activity-independent selection system of thermostable protein variants.
- Authors
Chautard, Hélène; Blas-Galindo, Emilio; Menguy, Thierry; Grand'Moursel, Laure; Cava, Felipe; Berenguer, José; Delcourt, Marc
- Abstract
We describe an activity-independent method for the selection of thermostable mutants of any protein. It is based on a fusion construct comprising the protein of interest and a thermostable antibiotic resistance reporter, in such a way that thermostable mutants provide increased resistance in a thermophile. We isolated thermostable mutants of three human interferons and of two enzymes to demonstrate the applicability of the system.
- Publication
Nature methods, 2007, Vol 4, Issue 11, p919
- ISSN
1548-7091
- Publication type
Journal Article
- DOI
10.1038/nmeth1090