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- Title
Subunit counting in membrane-bound proteins.
- Authors
Ulbrich, Maximilian H; Isacoff, Ehud Y
- Abstract
The subunit number and stoichiometry of membrane-bound proteins are difficult to determine without disrupting their membrane environment. Here we describe a single-molecule technique for counting subunits of proteins in live cell membranes by observing bleaching steps of GFP fused to a protein of interest. After testing the method with proteins of known stoichiometry expressed in Xenopus laevis oocytes, we resolved the composition of NMDA receptors composed of NR1 and NR3 subunits.
- Publication
Nature methods, 2007, Vol 4, Issue 4, p319
- ISSN
1548-7091
- Publication type
Journal Article
- DOI
10.1038/nmeth1024