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- Title
High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy.
- Authors
Verveer, Peter J; Swoger, Jim; Pampaloni, Francesco; Greger, Klaus; Marcello, Marco; Stelzer, Ernst H K
- Abstract
We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging large living multicellular specimens obtained in a three-dimensional cell culture. The ability to rapidly image large samples at high resolution with minimal photodamage provides new opportunities especially for the study of subcellular processes in large living specimens.
- Publication
Nature methods, 2007, Vol 4, Issue 4, p311
- ISSN
1548-7091
- Publication type
Journal Article
- DOI
10.1038/nmeth1017