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- Title
Allele-specific detection of single mRNA molecules in situ.
- Authors
Hansen, Clinton H; van Oudenaarden, Alexander
- Abstract
We describe a method for fluorescence in situ identification of individual mRNA molecules, allowing quantitative and accurate measurement, in single cells, of allele-specific transcripts that differ by only a few nucleotides. By using a combination of allele-specific and non-allele-specific probe libraries, we achieve >95% detection accuracy. We investigate the allele-specific stochastic expression of Nanog, which encodes a pluripotency factor, in murine embryonic stem cells.
- Publication
Nature methods, 2013, Vol 10, Issue 9, p869
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.2601